Autologous tissue regeneration with the Outin preparation involves the use of biologically activated patient plasma enriched with DNA nucleotides, which represents a "reservoir" of growth factors released from blood platelets - platelets. The method is used for:

 

PRP-"VAMPIRE FACELIFT" - DEFINITION

PRP-"platelet-rich plasma" or plasma rich in platelets is the patient's own blood with an increased concentration of platelets, which upon activation release growth factors (proteins), that is, it is a preparation obtained by centrifuging whole blood in order to increase the concentration of platelets.

Blood: number of platelets 150-350 x 10 9 in 1 mm3 of blood + anticoagulant.

PRP: soft spin: 1000 revolutions for 5-6 minutes.

Prf: 1300-2700 revolutions over 8-12 minutes.

PRP scientific works

The most commonly used blood derivatives in clinical use:

  • platelet rich plasma (prp): platelet rich plasma
  • platelet-rich growth factors (prgf)
  • platelet-rich fibrin (prf)
  • plasma enriched with platelets and leukocytes (l-prp)
  • fibrin enriched with platelets and leukocytes (l-prf), etc.

Platelets - history of discovery

Throughout history, before and after the scientific revolution, until today, scientists and naturalists have studied platelets, which allows us today to know a lot about platelet structure, function and the life of platelets in general. They were discovered by the French doctor Alfred Donne in 1842. Giulio Bizzozera, in 1882 established the fundamental knowledge about platelets, which were confirmed after him. Studying animal veins microscopically, he discovered that the shape of platelets is discoid, and that after an injury to a blood vessel in the circulation, the process of adhesion and aggregation begins: a role in the process of hemostasis. He was the first to define platelets as central cells of the clotting system. Before the discovery of platelets, Bizzozero also identified megakaryocytes in the bone marrow, which are also platelet precursors.

About 40 years ago, platelets were considered primarily cells responsible for hemostasis (stopping bleeding).

Thrombocytes are blood platelets, i.e. small, colorless corpuscles without a nucleus. There are 150-400 x109/1 mm3 of blood in a liter of blood. They are formed in the bone marrow from parts of megakaryocytes (cells with a large nucleus). They decompose quickly: their lifespan is from 3 to 7 days.

Photo: from left to right: erythrocyte, platelet, leukocyte

PRP concentrates platelets about five times.


What is special about platelets?

Platelets contain alpha granules that contain a large amount of growth factors and dense corpuscles. Alpha granules are released by the aggregation and adhesion of platelets at the site of injury or inflammation.

Abundance of granules in platelets

The most interesting property of platelets is the abundance of granules that contain a large number of biomolecules, among which is serotonin. We distinguish 3 types of granules: αalpha-granules, dense granules that are a powerful storage of serotonin (eng. Dens granules) and lysosomes. Since the 90s of the last century, scientific research and technology have shown that platelet GRANULES contain abundance of growth factors. Growth factors play a key role in the HEALING AND TISSUE REGENERATION PROCESS. Growth factors stimulate the migration and multiplication of stem cells.

The most interesting property of platelets is the abundance of granules that contain a large number of biomolecules, among which is serotonin. We distinguish 3 types of granules: αalpha-granules, dense granules that are a powerful storage of serotonin (eng. Dens granules) and lysosomes. Since the 90s of the last century, scientific research and technology have shown that platelet GRANULES contain abundance of growth factors. Growth factors play a key role in the HEALING AND TISSUE REGENERATION PROCESS. Growth factors stimulate the migration and multiplication of stem cells.

Schematic representation of the platelet structure

Growth factors

PDGF (platelet-derived growth factor) - a group of polypeptides that stimulate protein synthesis - especially collagen and angiogenesis

TGF beta, EGF, VEGF they stimulate angiogenesis and synthesis of collagen, cell matrix, fibronectin

PDEGF (platelet-derived epidermal growth factor) stimulates the proliferation of keratinocytes and fibroblasts

PDAF (platelet-derived angiogenesis factor) stimulates angiogenesis

IGF-1 stimulates the synthesis of cell matrix elements and the mitotic activity of stem cells

PF-4 (platelet factor 4) attracts fibroblasts

Activation of platelets and release of growth factors is what makes this therapy so widely applicable and interesting!

What is the role of growth factors?

CHEMOTAXY

MULTIPLICATION OF CELLS

Effect of growth factors on tissues

Immediate (within 5 minutes)

Early (30 minutes to 4 hours)

Late (4-24 hours)

Deferred (up to 3 months)

The overall effect of platelet-derived growth factors on tissues

Indications for application

Expected results

Preparation of platelet-rich plasma

Blood extraction

About 10-15 ml of blood is taken from the client into test tubes filled with anticoagulant (Na citrate).

Centrifugation

The blood is centrifuged at 1000 revolutions or 300g for 6 minutes, which separates the blood into 3 compartments: the upper and middle "buffycoat" rich in platelets and leukocytes, and the lower one rich in erythrocytes.

Separation of platelet-rich plasma

Platelet activation

Platelet activation is carried out with an activator containing calcium chloride, adenine, cytosine and thiamine

Application

Result of blood conditioning

On average, blood contains 2x 105 platelets per microliter, and centrifugation produces plasma rich in platelets, which clots up to 5 times higher concentration of platelets

Application methods

Injection techniques (SUPERFICIAL AND DEEP)

Papules: Surface intradermal technique, the application site is the junction of the epidermis and the dermis

Nappage: The technique was designed by Dalloz Bourguignon, the application is made with a series of injections at a depth of 2 to 4 mm, maintaining constant pressure in the syringe that is inserted into the skin at an angle of 30 to 60 degrees

Point by point: Deep intradermal and hypodermal technique at a depth of 4 mm and more

Microinjection techniques ("derma roller" and "derma pen")

Alpha granules and growth factors

Alpha granules release growth factors approx. 10 minutes after "activation" of platelets. The viability of platelets and the release of growth factors into the tissue continues until the end of the life of the platelet. In addition, platelets stimulate the influx of macrophages and stem cells, which additionally contribute to the process of tissue restoration and regeneration through the removal of degenerated and necrotic tissue, and stimulate tissue regeneration.

Growth factors stimulate regenerative processes: chemotaxis, proliferation, differentiation and angiogenesis.

PRF

PRP concentrates platelets about five times.

PRF represents a newer generation of platelet concentrates.

The special texture of PRF allows clinical use in amorphous and membrane form. The membrane form is used to cover and protect large tissue grafts. It stimulates osteogenesis and angiogenesis.


The first promotion of PRP

Dr. M. Ferrari 1987:

Open heart surgery and autologous blood component transfusion to avoid the use of homologous blood transfusion after open heart surgery.

Application from: orthopedics, sports medicine, dentistry, otolaryngology, neurosurgery, ophthalmology, urology, wound healing, aesthetic medicine, cardiothoracic and maxillofacial surgery.

PRP is an autologous product, the possibility of an immunogenic reaction and transmission of infectious diseases such as HIV and hepatitis is eliminated.

PRP – well-being

Recently documented beneficial effects of PRP on chronic non-healing injuries of tendons, ligaments, muscles, bones (osteoarthrosis), intervertebral disc degeneration, plantar fasciopathy and cartilage degeneration as well as in the care of muscle trauma and ischemic nerve damage.

PRP - effects

The growth factor found in plasma rich in transforming growth factor-β (TGF-β) stimulates:

PRP has received attention in the popular media as a result of its use in the treatment of sports injuries in professional athletes:

Tiger Woods

Born as Eldrick Tont Woods – American golfer. He is considered one of the greatest golfers of all time.

Raphael Nadal

Rafael "Rafa" Nadal Parera, professional Spanish tennis player. Nadal has won fourteen Grand Slam titles, the 2008 Olympic gold medal and 28 Masters 1000 titles and was a member of the Spanish Davis Cup team that won the titles in 2004, 2008, 2009 and 2011.

Kim Kardashian


"Vampire facelift"

Safety of use

Hypothesis: overexpression of growth factors and their receptors associated with the formation of tumors and tissue dysplasia.

Growth factors regulate various cellular processes, such as mitogenesis, chemotaxis, cell differentiation and metabolism. Neoplastic growth requires higher continuous doses of growth factors over a longer period of time. When applying PRP therapy, extracellular growth factors are broken down within 7-10 days.

Advantages of using: Autologous PRP - no risk of cross-reactivity - immune reaction or disease transmission.

Advantages of PRP compared to other methods of rejuvenation

Combining with other rejuvenation methods

PRP can be combined with:

Filers – Fillers lead to an immediate increase in volume, and injecting PRP above the filler improves the texture and tone of the skin and extends the effectiveness of the filler for 3 to 6 months

Laser therapy – Application of PRP after laser therapy significantly accelerates recovery and gives better final treatment results

Peelings – Accelerates healing after the application of medium-deep peels and improves the tone and texture of the skin

Absolute contraindications

Relative contraindications

Possible side effects

Practiced preparation and application is the key to success

As with other therapies, thorough training and experience are the most important prerequisites for an optimal treatment result!